Determination of loniceroside a and C in Lonicerae japonicae by RP-HPLC
Zhang, X.-G.; Wang, Z.-M.; Song, X.-M.; Zhu, J.-J.; Gao, H.-M.; Lin, L.-M.
Zhongguo Zhong Yao Za Zhi 32(20): 2149-2151; 2210
2007
ISSN/ISBN: 1001-5302 PMID: 18306750 Document Number: 610783
To develop an HPLC method for determination of loniceroside A and loniceroside C in Lonicerae japonicae. The analysis was carried out on an Alltech C18 column (4.6 mm x 250 mm, 5 microm) evaluated with methanol-acetonitrile-0.1% glacial acetic acid as mobile phase. Flow rate was at 1.0 mL x min(-1) and the detection wavelength was at 210 nm for UV detection. The calibration curves were linear over the range from 0.15 to 2.25 microg (r = 0.999 9) for loniceroside A and 0.11 to 1.65 microg (r = 0.999 1) for loniceroside C. The average recoveries were 99.9% and 98.3%, respectively. The contents of Loniceroside A&C are diverse in Flos Lonicerae japonicae in different regions.