Estrogens determination in urine by RP-HPLC with UV detection
Tirendi, S.; Lancetta, T.; Bousquet, E.
Farmaco 49(6): 427-430
1994
ISSN/ISBN: 0014-827X PMID: 8074785 Document Number: 427549
A new method for the determination of estrogens by high performance liquid chromatography with UV detection at 380 nm is reported. The estrogens after extraction with Bond-Elut C-18 cartridge were hydrolized, and derivatized with 7-chloro-4-nitro-1,3-benzoxadiazole in the presence of 18-crown-6 ether and K-2CO-3 and their derivatives were separated on a Hypersyl ODS 5 mu-m column with methanol-water (75:25 v/v) as the mobile phase. The relative standard deviation (RSD) of the method ranged from 2.1 to 3.2% and the average recovery was 98.1%.