Determination of residual clenbuterol in pork meat and liver by HPLC with electrochemical detection
Zhang, X-zhu.; Gan, Y-ru.; Zhao, F-nian.
Yao Xue Xue Bao 39(4): 276-280
2004
ISSN/ISBN: 0513-4870 PMID: 15303658 Document Number: 582522
Aim: This study was conducted to detect the residual clenbuterol in pork meat and liver using HPLC with coulometric electrode array system. Methods: Homogenized meat or liver samples were treated with 1 mol/litre hydrochloric acid and centrifuged. The fat present in meat or liver tissue was removed by diethyl ether. The pH of the remaining aqueous layer was adjusted to 10.8+or-0.2 or 11.6+or-0.2 for meat or liver, followed by liquid-liquid extraction with diethyl ether. The ether extract was evaporated to dryness and the residue was dissolved in the mobile phase. Mobile phase A consisted of 50 mmol/litre phosphoric acid-30 mmol/litre triethylamine and was adjusted to pH 4.0 with 2 mol/litre sodium hydroxide solution. Mobile phase B consisted of methanol-acetonitrile (30:45). A mixture of mobile phase A and B (80:20) was used. A 4-electrode array module was selected for quantitation with the electrode potentials set at 450, 600, 650, and 680 mV. Results: The 2 calibration curves for meat and liver showed good linearity between 1.88-60.16 ng/g, with a detection limit of 1.2 ng/g for clenbuterol. Conclusion: The method using HPLC-electrochemical detection is reproducible with a sensitivity good enough for the determination of clenbuterol in meat and liver.