Determination of the minimum enrichment time for the qualitative detection of Listeria monocytogenes in minced pork meat using multiplex-PCR, microarray and ELISA
Leidreiter, M.; Kühne, M.
Berliner und Munchener Tierarztliche Wochenschrift 120(1-2): 79-85
2007
ISSN/ISBN: 0005-9366 PMID: 17290946 Document Number: 613768
A qualitative detection of Listeria monocytogenes was performed in spiked minced pork meat using ELISA, Multiplex-PCR, Microarray and cultural reference method. Minced pork meat in batches of 10 or 25 g was spiked with 25 cfu Listeria monocytogenes per gram and incubated in selective enrichment solutions. During enrichment samples were collected continiously and a Listeria monocytogenes-ELISA, a Multiplex-PCR with electrophoretical detection (Listeria duplex) and a PCR with detection by Microarray (NUTRI Chip) were performed. The enrichment time after which all sub-samples were positive was defined as minimum enrichment time. With the cultural reference method Listeria monocytogenes was detected in 100 % of the samples after a total analysis time of 5 days. With the ELISA kit used in this study positive results were achieved after enrichment for 24 h. Multiplex-PCR with electrophoretical detection was positive after only 16 h of enrichment. The most sensitive detection method was the microarray. Using this technique, an enrichment time of 10 h was sufficient to get positive results in all samples.