Coupling of immunomagnetic separation and polymerase chain reaction for the rapid detection of salmonellae in minced meat and chicken giblets
Erol, I.; Kleer, J.; Hildebrandt, G.; Yurtyeri, A.
Berliner und Münchener Tierärztliche Wochenschrift 112(3): 100-103
1999
ISSN/ISBN: 0005-9366 PMID: 10209908 Document Number: 510595
Coupling of immunomagnetic separation and polymerase chain reaction was used after pre-enrichment for the rapid detection of salmonellae in minced meat and chicken giblets. The primers used are specific to the origin of DNA replication (oriC) on the Salmonella chromosome and produce a 163 bp DNA fragment. The method allowed screening of samples within 24 h. It detected 5 serovars when inoculated in minced meat at a contamination level of 1 to 4 c.f.u./g. Compared with the method L 00.00-20 of the Official Collection of Methods of Analysis according to Article 35 of the German Law on Food and Commodities it showed a sensitivity of 94% and a specificity of 87.5% for the examination of chicken giblets (45 samples) and a specificity of 100% for the examination of minced meat (pork and beef; 63 samples).