Experience with rapid molecular genetic diagnosis using the polymerase chain reaction
Macek, M.; Böhm, I.; Arnold, L.; Smrt, J.; Macek, M.; Duspivová, R.; Vávrová, V.; Sedlácek, Z.; Schmidtke, J.
Casopis Lekaru Ceskych 129(19): 590-595
1990
ISSN/ISBN: 0008-7335 PMID: 2350781 Document Number: 363836
The authors give an account of their experience with 1000 amplifications of DNA by the polymerase chain reaction for the prenatal diagnosis of cystic fibrosis. The method is demonstrated on examples of examinations of the informativity value and prenatal diagnosis in the first trimester of pregnancy in families with a 25% risk of cystic fibrosis, using J 3.11 (Msp I), met H (Msp I), KM 19 (Pst I), CS 7 (Hha I), Mp6d9 (Msp I), XV 2c (Taq I) probes. The authors summarize methodical check-up and safety measures to ensure the reliability of diagnoses made by the PCR method.