Determination of 3-methylhistidine in urine with HPLC

Caccialanza, G.; Gandini, C.; Kitsos, M.; Ponci, R.; Gazzani, G.

Il Farmaco; Edizione Pratica 43(4): 137-148

1988


ISSN/ISBN: 0430-0912
PMID: 3208878
Document Number: 308405
A new analytical method for the determination of 3-methylhistidine in urine has been developed. The method depends on HPLC revelation of the derivative of 3-methylhistidine with the reagent ninhydrin/OPA at 405 nm. Because of its reproducitability, accuracy and precision, the method is ideal for monitoring neuromuscular diseases.

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