Quantitative gas chromatographic determination of 3-methylhistidine in biological fluids
Rogozkin, V.A.; Krylov, A.I.; Meleshchenko, L.N.; Alekseev, A.T.
Ukrainskii Biokhimicheskii Zhurnal 58(4): 62-66
1986
ISSN/ISBN: 0201-8470 PMID: 3739032 Document Number: 271149
The gas chromatographic procedure is suggested to determine 3-methylhistidine in biological fluids. The amino acid fraction containing 3-methylhistidine is separated by ion-exchange chromatography. Amino acids are transformed into N-trifluoroacetyl-O-isobutyl esters which are analyzed by the gas chromatography instrument with micropacked columns and ionization-resonance detector. The limit of the quantitative determination of 3-methylhistidine is 50 ng per a probe.