Determination of tortoside a in Ilicis pubescentis by RP-HPLC
Li, S.; Zhou, Y.; Tu, P.
Zhongguo Zhong Yao Za Zhi 36(22): 3146-3148
2011
ISSN/ISBN: 1001-5302 PMID: 22375396 Document Number: 651850
To establish a RP-HPLC method for the determination of tortoside A in Ilex pubences. Kromasil-C18 (4.6 mm x 250 mm, 5 microm) column was used in HPLC with mobile phase acetonitrite-0.1% H3PO4 (17:83), the column temperature was 30 degrees C, the flow rate was 1 mL x min(-1), the detection wavelength was set at 210 nm, and inject volume was 10 microL RESULT: Tortoside A was well separated under the established conditions, the liner range of tortoside A was 26.05-521.00 microg (r = 0.999 9, n = 6), and the average recovery was 98.42%. It was the first time to establish the RP-HPLC method with accuracy, good reproducibility for determining the content of tortoside A in I. Pubescentis.