Determination of vetatramine in Veratrum nigrum by HPLC-ELSD
Wang, L.-S.; Zhao, D.-Q.; Tao, H.-M.; Liu, Y.-H.
Zhongguo Zhong Yao Za Zhi 33(7): 791-792
2008
ISSN/ISBN: 1001-5302 PMID: 18589783 Document Number: 615149
To develop an HPLC-ELSD method for determination of vetatramine. in Veratrum nigrum. The analy fical column was Shim-pack ODS - C18 (4.6 mm x 250 mm, 4 microm) column, the mobile phase was acetonitrile-water (containing 0.1% triethylamine) (50:50), at a flow rate of 0.8 mL x min(-1). The temperature of drift tube was 90 degrees C and the gas flow was at the rate of 2.5 L x min(-1). The calibration curve was linear in the range of 0.36-3.6 microg (r = 0.999 8). The average recovery was 100.9% (RSD 2.3%, n = 6). The contents of veratramine in Veratrum nigrum. from the ten different sources were determined. The method may be used as a accurate and reproducible way to determine the content of veratramine in V. nigrum.