Determination of astragulin in Thesium chinese by RP-HPLC
Liu, Y.; Deng, Y.-L.; Yu, Y.-H.; Dai, R.-J.; Xu, Z.-H.; Meng, W.-W.
Zhongguo Zhong Yao Za Zhi 31(21): 1766-1767
2006
ISSN/ISBN: 1001-5302 PMID: 17260787 Document Number: 604035
To establish a determination method of astragulin in Thesium chinese. RP-HPLC analytical method was established using a Polaris C18 column and acetonitrile-water (23:77) as the mobile phase, with a flow rate of 10 mL x min(-1), detected at 346 nm. The method of sample is refluxing exation by 50% alcohol for 2 times. The content of astragulin was from 0. 120% to 0. 155%, in different groups of T. chinese collected from the same location. The method was validated to show convenient and reliable.