Determination of twelve ginsenosides in Panax ginseng by HPLC
Xu, X.; Zheng, Y.; Fu, S.; Zhao, Y.; Li, J.; Wang, L.
Zhongguo Zhong Yao Za Zhi 36(11): 1463-1465
2011
ISSN/ISBN: 1001-5302 PMID: 22779178 Document Number: 651017
To determine the contents of twelve ginsenosides in the root of Panax ginseng by HPLC. The analysis is carried out at room temperature on a Luna NH2 column (4.6 mm x 150 mm, 5 microm) eluted with acetonitrile and water as the mobile phases in a gradient elution. The flow-rate was 1.0 mL x min(-1), the detection wavelength was 203 nm. Twelve ginsenosides (Rh2, Rh1, Rg2, Rg3, Rg1, Rf, Re, Rd, Re, Rb2, Rb3, Rb1) were separated at baseline within 60 min with good linearity (r > or = 0.999 5). The recovery rates were 98.1%, 95.3%, 96.1%, 95.6%, 97.3%, 98.6%, 98.0%, 96.4%, 96.1%, 97.6%, 96.8%, 96.9% (RSD < or = 3.0%). The method was simple,fast and could control the quality of P. ginseng effectively.