Simultaneous determination of panaxynol and panaxydol in fibrous root of Panax ginseng by HPLC
Li, J.; Jiang, J.; Zheng, Y.; Wang, L.; Yang, Y.; Hu, Y.
Zhongguo Zhong Yao Za Zhi 36(17): 2380-2382
2011
ISSN/ISBN: 1001-5302 PMID: 22121807 Document Number: 650154
To establish an HPLC method for simultaneous determination of panaxynol and panaxydol from the fibrous root of Panax ginseng. The analysis was performed on Elite C18 column (4.6 mm x 150 mm, 5 microm) with mobile phase gradient of CH3CN-water at a flow rate of 1.0 mL x min(-1). The detection wavelength was 230 nm, and the detection temperature was ambient. The linear range were 0.70-3.50 microg (r = 0.9995) for panaxynol, and 0.64-3.20 microg (r = 0.9999) for panaxydol. The average recoveries were 99.1% (RSD 1.7%) and 99.3% (RSD 1.2%), respectively. The HPLC method is simple, rapid and reproducible, which can be used for the quality control of the fibrous root of P. ginseng.