Determination of 5-hydroxy-7-methoxyflavan in Daemonorops draco by HPLC
Fu, M.; Fang, J.; Yang, H.; Wang, Z.; Tang, L.; Huang, L.; Yang, L.; Zhang, D.
Zhongguo Zhong Yao Za Zhi 35(23): 3192-3193
2010
ISSN/ISBN: 1001-5302 PMID: 21355247 Document Number: 644312
To establish a method for the determination of 5-hydroxy-7-methoxyflavan in Daemrnonorops draco by HPLC. Dikma Diamonsil C15 column(4. 6 mm x 150 mm, 5 microm) was used. The mobile phase consisted of (60:40). The temperature of column was 30 degrees C. The flow rate was 1:0 mL x min(-1) and the detection wavelength was at 210 nm. The calibration curve was in good linearity with in the range from 0.01 to 0.10 microg (r = 0.9999). The average recovery was 100.1% (n=6). The method is simple, rapid, as well as precise and reliable, and can be used for the determination of 5-hydroxy-7-methoxyflavan in D. draco.