HPLC determination of five constituents in plants of genus Ligusticum
Zhang, J.L.; He, X.F.; Zhou, Z.H.
Yao Xue Xue Bao 31(8): 622-625
1996
ISSN/ISBN: 0513-4870 PMID: 9772710 Document Number: 457198
A RP-HPLC method was established to separate and determine five constituents in plants of genus Ligusticum by using an ODS column (Hypersil-C-18, 5 mu-m) and acetonitrile (containing 1.25% CHCl-3) sbd water (saturated with CHCl-3) as mobile phase for gradient elution. The five constituents were ferulic acid, scopoletin, 3-butyl-phthalide, ligustilide, and diligustide. They were detected at 284 nm. Peak purity was monitored by photodiode array detector. Benzthiazide was used as the internal standard. The method is simple, fast, sensitive and reproducible.