Preparation of ferulic acid, senkyunolide i and senkyunolide H from Ligusticum chuanxiong by preparative HPLC
Xiong, Y.-K.; Liang, S.; Hong, Y.-L.; Yang, X.-J.; Shen, L.; Du, Y.; Feng, Y.
Zhongguo Zhong Yao Za Zhi 38(12): 1947-1950
2013
ISSN/ISBN: 1001-5302 PMID: 24066590 Document Number: 667686
Preparative HPLC was used to prepare ferulic acid, senkyunolide I and senkyunolide H from Ligusticum chuanxiong. The separation was conducted on a Shim-Pack Prep-ODS (20.0 mm x 250 mm, 5 microm) column with the mobile phase of methanol-0.2% glacial acetic acid (50:50)at the flow rate of 5 mL x min(-1). The detection wavelength was 278 nm, and the purity of each compound was detected by HPLC analysis. Spectral data analyses including UV, ESI-MS and NMR were used to identify their structures. This method is simple, fast, which is suitable for preparation of standard reference of ferulic acid, senkyunolide I and senkyunolide H from L. chuanxiong and can meet the requirement of new drug research and development.