Preparation of three capsaicinoid components using preparative high performance liquid chromatography
Dong, X.; Liu, Z.; Li, B.; Wang, K.
Se Pu 26(3): 366-369
2008
ISSN/ISBN: 1000-8713 PMID: 18724678 Document Number: 627723
A method was established for the isolation of three capsaicinoid components from capsaicinoids using preparative high performance liquid chromatography (Pre-HPLC). A preparative PRC-ODS column (250 mm x 21.5 mm, 13 microm) was used in the preparation process. By isocratic elution with methanol-water (70 : 30, v/v) as the mobile phase at a flow rate of 15 mL/min followed by concentration, three capsaicinoid components were obtained from 80% capsaicinoids, and subsequently identified by 1H NMR (nuclear magnetic resonance) and EI-MS (electron impact-mass spectrometry) as nordihydrocapsaicin, capsaicin and dihydrocapsaicin, and the purities of the three components analyzed by HPLC were 98.12%, 99.93% and 100.7% with yields of 60.1%, 58.9% and 72.3%, respectively.