Reverse phase analytical and preparative high pressure liquid chromatography of aflatoxins
Stubblefield, R.D.; Shotwell, O.L.
Journal - Association of Official Analytical Chemists 60(4): 784-790
1977
ISSN/ISBN: 0004-5756 PMID: 408324 Document Number: 120351
Reverse phase high pressure liquid chromatography (HPLC) was investigated and found applicable for determining 5 aflatoxins. Aflatoxins M2, M1, G2, and G1 and B1 were completely resolved and B2 was satisfactorily separated on a C18 (10 .mu.m, 4 mm id .times. 8') developed with acetonitrile-water mixtures (M1, 20 + 80; B1, 35 + 65; G1, 25 + 75) at 9.0 ml/min. These columns permitted isolation of 40 mg aflatoxin B1 or G1 in less than 3 h. Aflatoxin M1 required 2 HPLC steps.