Genetic engineering of human insulin. IV. Development and optimization of an analysis system using reversed phase high pressure liquid chromatography

Kliushnichenko, V.E.; Iakimov, S.A.; Arutiunian, A.M.; Ivanov, A.E.; Mal'tsev, K.V.; Vul'fson, A.N.; Kliushnichenko, V.E; Iakimov, S.A; Arutiunian, A.M; Ivanov, A.E; Mal&apo; tsev, K.V; Vul&apo; fson, A.N

Home

>

Node

>

Subnode

Genetic engineering of human insulin. IV. Development and optimization of an analysis system using reversed phase high pressure liquid chromatography

Kliushnichenko, V.E.; Iakimov, S.A.; Arutiunian, A.M.; Ivanov, A.E.; Mal'tsev, K.V.; Vul'fson, A.N.

Bioorganicheskaia Khimiia 20(10): 1080-1088

1994

ISSN/ISBN: 0132-3423

PMID: 7826407

Document Number: 429333

The effectiveness of the RP HPLC application for the step-by-step analysis of the recombinant insulin production was studied. Properties of a number of commercial and experimental columns in different chromatographic conditions were considered. A three-dimension optimization of selectivity and resolution versus pH and ion strength was carried out. A mechanism of the resolution and selectivity control is suggested.