Determination of propranolol in human plasma by reversed phase high performance liquid chromatography
Yuan, Y.S.; Xing, X.F.; Zeng, P.; Zhou, X.
Yao Xue Xue Bao 22(3): 238-240
1987
ISSN/ISBN: 0513-4870 PMID: 3661213 Document Number: 286557
This paper investigates the determination method of propranolol in human plasma by HPLC. YWG-C18H37 column was used with CH3OH-H2O-0.2 mol/L KH2PO4 0.2 mol/L Na2HPO4 (460:40:0.7:4.7) as mobile phase. The preparation of plasma sample: Transfer 0.5 ml plasma into a 5 ml polystyrene test tube with cap, add 0.8 ml of 0.2% sodium hydroxide solution into the plasma. Propranolol was extracted with ether. External standard was used for quantitation. The assay was evaluated using the reproducibility within day and day-to-day, and demonstrated high precision. Their average coefficients of variation were 5.8% and 6.8% respectively. The standard curve was linear (r 0.9999) over the range of 0.05 .apprx. 2.00 .mu.g/ml, the average recovery was 95.9 .+-. 4.5%.