Determination of glycyrrhetic acid in toothpaste by reversed-phase high performance liquid chromatography (RP-HPLC)
Li, L.
Se Pu 17(5): 493-494
1999
ISSN/ISBN: 1000-8713 PMID: 12552894 Document Number: 500367
A method for determining glycyrrhetic acid in toothpaste by RP-HPLC is described. The operating conditions were YWG-C18 column (4.0 mm i.d. x 250 mm, 10 microns) at room temperature, V(methanol):V(0.01 mol/L KH2PO4) = 85:15 (pH 3.0) as mobile phase with flow rate of 1.0 mL/min and UV detection at 254 nm. Glycyrrhetic acid in toothpaste samples were extracted with methanol. The average recoveries of glycyrrhetic acid were 99.61%-101.67%. The relative standard deviations were 1.85%-3.16%. The method is simple, rapid and accurate.