Determination of berberine hydrochloride in traditional Chinese medicines containing Coptis chinensis Franch by reversed phase high performance liquid chromatography
Guo, P.; Li, Z.; Hong, Z.; Liu, S.; Wu, T.
Hua Xi Yi Ke da Xue Xue Bao 22(1): 90-92
1991
ISSN/ISBN: 0257-7712 PMID: 1774045 Document Number: 369902
This paper reports a reversed phase high performance liquid chromatography method to determine berberine hydrochloride in Coptis chinensis Franch and in the traditional Chinese medicine cotaining Coptis chinensis Franch. The result shows that the mobile phase of 0.4 mol/L ammonium nitrate-methanol (52:48) can give a satisfactory separation. The extraction of ample with 1% H2SO4 solution using the ultrasonic vibration method is simple and easy to operate.