Separation of different phosphatidyl cholines by reversed-phase ion-pair high performance liquid chromatography
Wang, Z.-h.; Lu, X.-q.; Hong, X.-k.
Se Pu 20(3): 249-252
2002
ISSN/ISBN: 1000-8713 PMID: 12541948 Document Number: 551298
A reversed-phase ion-pair high performance liquid chromatographic (RP-IP-HPLC) method for the separation of different phosphatidyl cholines (PC) was established. The optimized conditions were a PERKIN-ELMER/HS-5 C18 column (150 mm x 4.6 mm i.d., 5 microns), isocratic elution with mobile phase of methanol-acetonitrile-water (70:22:8, volume ratio) containing 15 mmol/L tetramethyl ammonium phosphate (TMAP) at pH 7.0, flow rate of 2.0 mL/min and UV detection at 208 nm. Seven kinds of PC could be separated with this method.