HPLC determination of salidroside in the roots of Rhodiola genus plants
Wang, S.; You, X.T.; Wang, F.P.
Yao Xue Xue Bao 27(11): 849-852
1992
ISSN/ISBN: 0513-4870 PMID: 1300030 Document Number: 389672
This paper reports a high performance liquid chromatographic (HPLC) method for the determination of salidroside in 10 samples of the plants of Rhodiola crenulata, R. alterna, R. brevipetiolata, R. yunnanensis, and R. quadrifida as well as extracts of R. crenulata. Determination was carried out under the following conditions: column (mu-Bondapak ODS, 10 mu-m, 3.9 mm times 300 mm), mobile phase (methanol-water, 2:8), flow rate (1.0 ml/min), detector (Waters 484, wavelength 276 nm). External standard method was used. The results show that HPLC is a useful tool for the quantitative analysis of salidroside in the plants of Rhodiola genus and the extracts of R. crenulata.