Determination of salidroside and p-tyrosol in Hongjingtian for injection (freezing-dry) by SPE-HPLC
Meng, J.; Wang, S.-F.; Han, F.; Li, S.-M.
Zhongguo Zhong Yao Za Zhi 31(2): 119-121
2006
ISSN/ISBN: 1001-5302 PMID: 16570796 Document Number: 603243
To assay salidroside and p-tyrosol in Hongjingtian for injection (freezing-dry). Samples were purified by Sep-Pak C18 column and salidroside and p-tyrosol were determined by HPLC with Irregular-H C18 (4.6 mm x 250 mm, 5 microm), and eluted with a mobile phase of methanol-acenitonitrile -0.06% phosphate (10: 10:80). The flow rate was 1.0 mL x min(-1), the detection wavelength was set at 275 nm and the column temperature was maintained at 30 degrees C. The calibration curves were linear in the range of 2.24-22.4 microg for salidroside (0.999 7) and 0.856-8.56 microg for p-tyrosol (0.999 6), the average recovery was 101.3%, 99.8% respectively. The method is convenient, rapid, accurate and reliable.