Determination of antitubercular drugs in human plasma by HPLC through direct injection
Altomare, C.; Carotti, A.; Cellamare, S.; Leogrande, G.; De Vito, D.
Farmaco 45(11): 1229-1236
1990
ISSN/ISBN: 0014-827X PMID: 2088365 Document Number: 351337
A simple and efficient method is described for the direct determination of Isoniazide (INH) and Pyrazinamide (PZAm) in human plasma, based on reversed-phase HPLC. A number of other antitubercular drugs such as p-Aminosalycylic acid (PAS), Streptomycin (STM) and Ethambutol (ETH) and the major metabolites of the two investigated drugs, namely N-Monoacetylisoniazide (AcINH) and Pyrazinoic acid (PZAc), were shown not to interfere with the assay method. A potential application of the method of routine clinical monitoring of antitubercular drugs is discussed.