Determination of aprindine in human plasma using reversed phase HPLC
Misztal, G.; Przyborowski, L.
Die Pharmazie 50(3): 187-188
1995
ISSN/ISBN: 0031-7144 PMID: 7732048 Document Number: 446060
A rapid, simple, accurate method is presented for the determination of aprindine in human plasma. Liquid-liquid extraction of aprindine was carried out using diethyl ether. Amiodarone was applied as an internal standard. The samples were chromatographed on LiChrosorb RP-18 (10 microns) column and the mobile phase was methanol/acetonitrile/phosphate buffer pH 2.5 (80:15:5). The detection was carried at 254 nm. The method was tested for linearity (range from 0.5 to 2.5 micrograms/ml), recovery (ca. 90%) and precision (CV = 3.7%).