Evaluation of water-soluble vitamins in the vitamin preparation Spofavit dragée using reversed-phase HPLC
Jedlicka, A.; Klimes, J.
Ceska a Slovenska Farmacie Casopis Ceske Farmaceuticke Spolecnosti a Slovenske Farmaceuticke Spolecnosti 53(5): 243-247
2004
ISSN/ISBN: 1210-7816 PMID: 15506708 Document Number: 575651
A reversed-phase HPLC gradient method with good separation efficiency was developed and validated for the evaluation of water-soluble vitamins in the pharmaceutical preparation Spofavit dragee. Analyses were performed on a C8 column (LiChrospher RP-select B), the mobile phase was a mixture of phosphate buffer with sodium hexanesulphonate and acetonitrile. Detection was at 280 and 210 nm. The method is capable within 7 minutes to evaluate 6 water-soluble vitamins (ascorbic acid, nicotinamide, panthotenate.Ca, pyridoxine.HCl, thiamine.HCl, and riboflavin). All validated parameters of this method are discussed; the developed method appeared robust and suitable for evaluation of water-soluble vitamins in vitamin preparations.