The effects of water-soluble vitamins on the expansion of rabbit blastocysts in vitro
Kane, M.T.
Journal of Experimental Zoology 245(2): 220-223
1988
ISSN/ISBN: 0022-104X PMID: 2966848 DOI: 10.1002/jez.1402450211Document Number: 478317
The vitamin requirements for culture of rabbit morulae to expanded blastocysts were examined. Early morulae were cultured for 5 days either in a control complete medium containing all the 11 water-soluble vitamins of F10 culture medium (biotin, pantothenate, choline, inositol, niacinamide, pyridoxine, riboflavin, thiamine, folic acid, B12, and lipoic acid) or in media with each vitamin omitted individually. Blastocyst diameters were measured at the end of culture. The omission of inositol, pyridoxine, riboflavin, and niacinamide resulted in large statistically significant decreases in blastocyst expansion. The omission of B12 resulted in a significant increase in blastocyst expansion indicating that the level present in F10 is toxic to rabbit blastocysts.
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