Determination of Plasma Amino Acids by Fluorescent Derivatization and Reversed-Phase Liquid Chromatographic Separation
Badiou, S.; Lehmann, S.; Jean-Cristol, P.; Bellet, H.
Clinical Laboratory 50(3-4): 153-158
2004
ISSN/ISBN: 1433-6510 PMID: 15074468 Document Number: 8687
Amino acid analysis in physiological fluids remains expensive, as it usually requires a dedicated analyzer. We modified an RP-HPLC method originally devoted to peptide and protein hydrolysate analysis in order to apply it to plasma amino acid determination. The described method uses a commercial kit based on 6-aminoquinolyl-Nhydroxysuccinimidyl carbamate derivatization followed by reversed-phase high-performance liquid chromatography. The described method is easy to use and allows the determination of twelve amino acids frequently involved in aminoacidopathies, thus enabling the diagnosis and follow-up of affected patients. The results obtained by this method are comparable to those obtained with a classical amino acid analyzer.
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