Concentration of sodium ion as the determining factor for the association of dansyl amino acids with the teicoplanin molecule in reversed-phase liquid chromatography
Ismaili, L.; Truong, T.Than.; André, C.; Thomassin, M.; Mozer, J-Louis.; Robert, J-Francois.; Xicluna, A.; Refouvelet, B.; Millet, J.; Nicod, L.; Guillaume, Y-Claude.
Journal - Association of Official Analytical Chemists 86(2): 222-228
2003
ISSN/ISBN: 0004-5756 PMID: 12723909 Document Number: 553133
The mechanism of the binding of D,L dansyl amino acids to teicoplanin was investigated. Na+ was used as an indicator of the interactions between the solutes and teicoplanin. The number (n) of sodium ions, Na+, excluded from the solute-teicoplanin interface when analyte transfer occurred was determined. A thermodynamic study and enthalpy-entropy compensation were performed to further explore the interaction mechanism. From these results, it was shown that teicoplanin was balanced between 2 conformational states characterized by distinct enantioselective properties. This approach indicates that liquid chromatography (LC) is a useful tool to extract physicochemical and molecular information from retention data. Thus, LC can be used as a complementary technique with the conventional techniques of molecular interaction analysis.