Measurement of gliclazide in plasma by radial compression reversed-phase liquid chromatography
Charles, B.G.; Ravenscroft, P.J.
Clinical Chemistry 30(11): 1789-1791
1984
ISSN/ISBN: 0009-9147 PMID: 6488522 Document Number: 229136
We describe a rapid, specific, and precise analysis for gliclazide in plasma by radial compression, reversed-phase, "high-performance" liquid chromatography. Gliclazide and the internal standard, 3-chlorogliclazide, are eluted after 4.4 and 6.8 min, respectively. Only 100 microL of plasma and minimal sample workup are required. The limit of detection for gliclazide in plasma is 0.5 mg/L (1.55 mumol/L) at 229 nm. Precision (CV) of the assay for 10 and 1 mg of gliclazide per litre is 2.1% and 6.4%, respectively.