Preparation of 40S ribosomal subunit proteins of rat liver by combination of ion exchange chromatography with reversed phase liquid chromatography
Dettmer, R.; Kärgel, H.J.; Stahl, J.
Biomedica Biochimica Acta 43(7): 937-945
1984
ISSN/ISBN: 0232-766X PMID: 6517889 Document Number: 235355
Total protein of small subunits of rat liver ribosomes was fractionated by chromatography on carboxymethylcellulose. Aliquots (between 5 and 30 mg) of the protein material of each peak were further separated by reversed phase liquid chromatography on an octadecasilyl silica column. In this way 15 of the 32 proteins of the small ribosomal subunit of rat liver could be isolated in milligram amounts.