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Purification of ribosomal proteins from Escherichia coli by cation exchange and reversed phase FPLC

Tam, M.F.; Giri, L.

Biochemistry International 11(5): 709-718

1985

ISSN/ISBN: 0158-5231
PMID: 3911957
Document Number: 242422
A complex mixture of 21 proteins from the 30S ribosomal subunit of Escherichia coli was fractionated on a cation-exchanger, then further separated on a C8 reversed-phase column. A set of 14 proteins were purified to homogeneity. The same protein mixture was also analysed on a C8 RPC column using a triethylamine phosphate (TEAP, pH2.2)/acetonitrile or a trifluoroacetic acid/acetonitrile solvent system which gave 11 and 8 purified proteins, respectively. Altogether, 16 out of 21 proteins from the 30S ribosomal subunit were purified.

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