Studies on the packings of high performance strong cation exchange chromatography for the separation of proteins
Chang, J.; Guo, Z.
Se Pu 15(4): 352-353
1997
ISSN/ISBN: 1000-8713 PMID: 15739476 Document Number: 478202
In this paper, the studies on the synthesis, chromatographic characteristics and application of new packings of high performance strong cation exchange chromatography with sulfonic group for the separation of proteins have been reported. The silica with the end hydroxyl group reacted with chlorosulfonic acid and thus packings for high performance strong cation exchange chromatography were obtained. The recovery of bioactivity for lysozyme of these high resolution packings is over 76%. The packings can be used for the separation of proteins and biotechnological products.