Separation of carbohydrates and carbohydrate derivatives by HPLC with cation-exchange columns at high pH
Togami, D.W.; Poulsen, B.J.; Batalao, C.W.; Rolls, W.A.
Biotechniques 10(5): 650-655
1991
ISSN/ISBN: 0736-6205 PMID: 1910782 Document Number: 371074
Cation-exchange columns were found to be stable when used at high pH and high temperature for high-performance liquid chromatographic separations of carbohydrates and carbohydrate derivatives. Pulsed amperometric detection and refractive index detection were found to be suitable detection modes with these cation-exchange columns. Significant differences in carbohydrate separation selectivity were observed between cation-exchange and anion-exchange columns.