Chromatographic separation of ribonucleic acids from bacterial and animal cells on MAB columns
Shirobokov, V.P.; Azikova, T.P.; Zagoriuko, E.E.
Biokhimiia 40(3): 531-537
1975
ISSN/ISBN: 0320-9725 PMID: 1203371 Document Number: 93828
Practicable and efficient method of the chromatographic separation of ribonucleic acids from animal and bacterial cells on columns with MAB-methylated albumin adsorbed on bentonite basis is developed. Distinct separation of transport and ribosomal RNAs was obtained on MAB columns. Ribosomal RNA from bacterial cells was, in its turn, separated in two fractions. Effect of pH, elution rate and temperature on the efficiency of the fractionation is studied. The method proposed is shown to have a number of advantages as compared with well-known method of RNA fractionation on MAK columns. The essence of the method described is irreversible inactivation of RNAse by AMB, which is confirmed in the series of special experiments.