Top Abstract Purchase Related
Home
>
Node
>
Subnode

E.S.R. of spin-trapped radicals in gamma-irradiated polycrystalline amino acids. Chromatographic separation of radicals

Makino, K.; Riesz, P.

International Journal of Radiation Biology and Related Studies in Physics Chemistry and Medicine 41(6): 615-624

1982

ISSN/ISBN: 0020-7616
PMID: 6288602
Document Number: 196427
The free radicals produced by gamma-radiolysis of polycrystalline amino acids (L-valine, L-leucine, L-isoleucine and L-proline) at room temperature in the absence of air were investigated by spin trapping with 2-methyl-2-nitrosopropane (MNP). The spin adducts produced by dissolving the irradiated solids in aqueous MNP solutions were separated by high-performance liquid chromatography and then identified by e.s.r. Deamination (ring-opening reaction for L-proline) was observed for all amino acid. For L-valine and L-leucine, H-abstraction from the tertiary carbon in the side chains occurred. For isoleucine, H-abstractions from the alpha-carbon of the amino acid and from a non-terminal carbon in the side chain were found.

Document emailed within 1 workday
Secure & encrypted payments

Lion, Y.; Denis, G.; Mossoba, M.M.; Riesz, P. 1983: E.s.r. of spin-trapped radicals in gamma-irradiated polycrystalline DL-alanine. A quantitative determination of radical yield International Journal of Radiation Biology and Related Studies in Physics Chemistry and Medicine 43(1): 71-83
Rustgi, S.; Joshi, A.; Moss, H.; Riesz, P. 1977: E.s.r. of spin-trapped radicals in aqueous solutions of amino acids. Reactions of the hydroxyl radical International Journal of Radiation Biology and Related Studies in Physics Chemistry and Medicine 31(5): 415-440
Rustgi, S.; Joshi, A.; Riesz, P.; Friedberg, F. 1977: E.s.r. of spin-trapped radicals in aqueous solutions of amino acids. Reactions of the hydrated electron International Journal of Radiation Biology and Related Studies in Physics Chemistry and Medicine 32(6): 533-552
Namiki, M.; Hayashi, T.; Ohta, Y. 1977: Novel free radicals formed by the amino-carbonyl reactions of sugars with amino acids, amines, and proteins Advances in Experimental Medicine and Biology 86b: 471-501
Colombetti, G.; Monti, S. 1972: E.P.R. of radiation-induced radicals in A- and Se-amino acids International Journal of Radiation Biology and Related Studies in Physics Chemistry and Medicine 21(4): 321-327
Pozniak, A.L.; Arzhankov, S.I.; Shagisultanova, G.A. 1974: Free radicals during photooxidation of amino acids and dipeptides sensitized by iron (III) complexes Biofizika 19(2): 233-237
Shtarkman, I.N.; Gudkov, S.V.; Chernikov, A.V.; Bruskov, V.I. 2008: Formation of hydrogen peroxide and hydroxyl radicals in aqueous solutions of L-amino acids by the action of X-rays and heat Biofizika 53(1): 5-13
Ahlbehrendt, I. 1976: A simple thin-layer chromatographic method for the separation of amino acids in urine Zeitschrift für Medizinische Labortechnik 17(3): 143-145
Badiou, S.; Lehmann, S.; Jean-Cristol, P.; Bellet, H. 2004: Determination of Plasma Amino Acids by Fluorescent Derivatization and Reversed-Phase Liquid Chromatographic Separation Clinical Laboratory 50(3-4): 153-158
Il'iasov, A.V. 2013: Phosphorus-containing iminoxy and nitroxy free radicals as perspective spin labels Biofizika 58(2): 233-238
Zhang, L.; You, J.; Ping, G.; Zhang, W.; Yan, C.; Zhang, Y. 2004: Micellar electrokinetic capillary chromatographic separation of amino acids derivatized with 2-(9-carbazole)-ethyl chloroformate as a pre-column labeling reagent Se Pu 22(2): 166-169
Dan, N.; Li, X.J.; Zhao, B.L.; Zhang, T.M.; Xin, W.J. 1989: Scavenging effects of probimane on active oxygen free radicals by electron spin resonance Zhongguo Yao Li Xue Bao 10(5): 443-447
Hadzija, O.; Keglević, D. 1977: Simple method for the separation of amino acids, amino sugars and amino alcohols related to the peptidoglycan components on a standard amino acid analyser Journal of Chromatography 138(2): 458-460
Shimada, M.; Kihara, T.; Kurimoto, K.; Maruyama, Y.; Ijichi, H. 1972: Topographic separation of lyophilized mouse brain and gas-liquid chromatographic analysis of free amino acids in brain divisions Kaibogaku Zasshi. Journal of Anatomy 47(2): 138-153
Chen, L.; Zang, Y.; Zhu, M.; Barr, B.; Mo, J.; Wang, D. 1992: Direct measurement of oxygen free radicals following cardiac failure of ischemic dog myocardium with the electron spin resonance technique Canadian Journal of Cardiology 8(10): 1066-1070
Miyake, M.; Fuchimoto, S.; Iwagaki, H.; Matsubara, N.; Edamatsu, R.; Hiramatsu, M.; Orita, K. 1991: Production of hydroxyl radicals by tumor cells varies with cell type as measured by electron spin resonance spectrometry Research Communications in Chemical Pathology and Pharmacology 71(3): 293-307
Hotz, G. 1973: Infectious DNA from coliphage T1. IV. The action of radicals induced by 60Co-gamma rays in the wet state International Journal of Radiation Biology and Related Studies in Physics Chemistry and Medicine 24(1): 1-13
Shanaida, M.; Kernychna, I.; Shanaida, Y. 2017: Chromatographic Analysis of Organic Acids, Amino Acids, and Sugars in Ocimum Americanum L Acta Poloniae Pharmaceutica 74(2): 729-734
Launois, P.; Dieye, A.; Blum, L.; Millan, J.; Sarthou, J.L. 1989: Macrophage activation and Mycobacterium leprae: effect of interferon gamma on the production of oxygen free radicals by phagocytic cells Dakar Medical 34(1-4): 78-82
Wiezorek, C. 1984: Comparison of radiation-induced radical yields in short peptides and constituent amino acids by detection of trapped oxygen isotope 18O International Journal of Radiation Biology and Related Studies in Physics Chemistry and Medicine 45(1): 93-97