Direct measurement of oxygen free radicals following cardiac failure of ischemic dog myocardium with the electron spin resonance technique
Chen, L.; Zang, Y.; Zhu, M.; Barr, B.; Mo, J.; Wang, D.
Canadian Journal of Cardiology 8(10): 1066-1070
1992
ISSN/ISBN: 0828-282X PMID: 1337727 Document Number: 401907
Objective: To determine whether the involvement of oxygen-derived free radicals in the pathogenesis of ischemic cardiac failure in dogs. Design: Free radicals were measured in normal and ischemic myocardium from dogs with cardiac failure induced by acute coronary occlusion. The changes in free radical production in ischemic myocardium (compared with normal myocardium with or without polymorphonuclear leukocytes) were studies, with plasma superoxide dismutase and malondialdehyde analyzed concurrently. Main results: The results showed that the oxygen free radicals in the ischemic myocardium were about 42.5% higher than those in the normal myocardium, about 67.8% higher than those in the normal myocardium washed with normal saline and about 60.5% higher than those in the ischemic myocardium washed with normal saline (P lt 0.05). Compared with controls, the concentration of malondialdehyde in ischemic dog myocardium was increased markedly. The activity of plasma superoxide dismutse was not significantly different between control dogs and dogs with ischemic cardiac failure. Conclusions: The current results indicate that there was increased production of free radicals and peroxidative damage in ischemic myocardium of dogs with cardiac failure. Polymorphonucler leukocytes apper to be a main source of free radicals in dogs with ischemic cardiac failure.