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Separation by high-performance liquid chromatography of phenylthiohydantoin derivatives of amino acids released by Edman degradation

Bollet, C.; Caude, M.

Journal of Chromatography 121(2): 323-328

1976

ISSN/ISBN: 0021-9673
PMID: 932149
Document Number: 96269
The separation by high-performance liquid chromatography of seventeen phenylthiohydantoin derivatives of amino acids released after Edman degradation of proteins is described. Sixteen phenylthiohydantoin derivatives of amino acids are differentiated within 40 min by liquid-liquid partition on a column packed with Micropak CN (moderately polar alkyl nitrile phase bonded on 10mum porous silica microparticles) and gradient elution with hexane=methylene chloride-isopropanol mixtures.

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