Separation of hemoglobins F, Fac, S, C, A1c and determination of hemoglobin F using high performance liquid chromatography
Pic, P.; Ducrocq, R.; Girot, R.
Annales de Biologie Clinique 52(2): 129-132
1994
ISSN/ISBN: 0003-3898 PMID: 7528482 Document Number: 433883
Separation of hemoglobins F, Fac, S, C and A1c was performed using high performance liquid chromatography with a cation exchange Polycat A column in a 15-minute assay. HbF titration results were well correlated with those of the reference alkali denaturation technique for values below 12% (r = 0.95; P < 0.001). This technique may be used as a confirmation test for neonatal screening of sickle cell disease.