The expression of tumor suppressor gene p53 and Rb gene in nasopharyngeal carcinoma
Song, Y.; Wang, L.; Huang, G.; Huang, R.; Yuan, S.; Liu, Y.
Hua Xi Yi Ke da Xue Xue Bao 28(3): 268-271
1997
ISSN/ISBN: 0257-7712 PMID: 10684031 Document Number: 478203
In order to observe the relation of tumor supperssor gene p53 and Rb gene to nasopharyngeal carcinoma, we investigated p53 gene mutations in exon 7-8 in 33 cases of nasopharygeal carcinoma (NPC), using single-strand conformation ploymorphism analysis of PCR (PCR-SSCP). The data showed p53 mutations in 7 of 33 specimens at exon 8 (21.2%). No mutation in exon 7 was detected. Analysis of Rb gene in NPC tissues by southern blot revealed the deletion and loss of activity of Rb gene in 11 of 13 cases (86.6%). Our results suggest that Rb gene and p53 gene are closely associated with the tumorigenesis of NPC.