Expression of metastasis-suppressor gene nm23H1 product in nasopharyngeal carcinoma
Huang, G.; Zeng, X.; Lu, Y.; Ning, Q.; Yang, C.; Jiang, Y.
Hua Xi Yi Ke Da Xue Xue Bao 28(1): 40-44
1997
ISSN/ISBN: 0257-7712 PMID: 10684060 Document Number: 483892
Using the specific monoclonal antibody of nm23H-1 gene product and the immunohistochemical technique, we studied the expression of DNPK/nm23H-1 and its correlation with lymph-node metastasis in nasopharyngeal carcinoma (NPC). The results revealed that, in a total of 31 cases of NPC, 13 showed positive expression with a positive rate of 41.9%. Among 21 cases of NPC with no lymph-node metastasis, 11 (52.3%) showed positive expression while only 20% (2/10) of those with lymph-node metastasis were positive, and there was significant difference between them (P lt 0.01). Meantime the expression was very low or not detectable in the cancerous cells of the match lymph-node metastasis and all 10 cases showed negative expression. Statistical significant difference was found between the group of NPC with no metastasis and the group of lymph-node metastasis (P lt 0.01). The above results indicated that high expression of DNPK/nm23H-1 existed in the group of NPC with no metastasis while low expression existed in those with metastasis and lymph-node metastasis. It suggests that the expression of nm23H-1 is inversely associated with lymph-node metastasis. The DNPK/nm23H-1 may play a role in polymerization of microtubulin protein and participate in the process of cancer metastasis.