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Localization of Epstein-Barr virus in lymph node metastasis with nasopharyngeal carcinoma

Akao, I.; Sato, Y.; Mukai, K.; Sugano, S.; Hoshikawa, T.; Suzuki, T.; Tsutsumi, K.

Acta Oto-Laryngologica. Supplementum 522: 86-88

1996

ISSN/ISBN: 0365-5237
PMID: 8740817
Document Number: 458868
Epstein-Barr virus DNA was detected in 3 cases of lymph node metastasis with nasopharyngeal carcinoma, using in situ hybridization. EBV-DNA was demonstrated on the nuclei of the neoplastic cells, and was absent on those of lymphocytes. Localization of EBV-DNA in neoplastic cells was confirmed by the double-labeling method combining in situ hybridization and immunocytochemistry. Direct evidence of the presence of EBV-DNA in malignant epithelial cells from lymph node metastasis with nasopharyngeal carcinoma was clearly demonstrated in this study.

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