Allergenic fractions of bacteria belonging to the enteric family. VII. Characteristics of the intracellular proteins comprising the allergenic substrate of Escherichia coli using ion exchange chromatography
Maianskiĭ, A.N.; Molchanova, I.V.; Alatyrtseva, I.E.
Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 3: 28-32
1976
ISSN/ISBN: 0372-9311 PMID: 785905 Document Number: 97831
A method of successive chromatography on Sephadex G-50 and G-100 and DEAE-Sephadex A-50 was applied for separation of the allergenic complex of E. coli intracellular proteins into a number of immunochemically different or significantly differing fractions with a definite set of antigens. Results of analysis of the fractions obtained in the tests of increased sensitivity of delayed type (ISDT) in vivo and in vitro corresponded to the view of the immunological polyvalency of the allergenic substrate of enterobacteria. Four groups of antigens were distinguished by the ratio of the activity indices in the skin reactions of ISDT and in the tests of specific inhibition of macrophage migration. Singificance of individual antigens in the formation of the allergenic profile of bacterial cells is determined by their quantitative content and the extent of the allergenic activity.