Intracellular development of Escherichia coli bacteriophages after culturing the infected bacteria under conditions of anabiosis and hypothermia
Tsutsaeva, A.A.; Vysekantsev, I.P.; Mikulinskiĭ, I.E.
Mikrobiologiia 55(6): 1009-1013
1986
ISSN/ISBN: 0026-3656 PMID: 3547045 Document Number: 283449
The intracellular growth of bacteriophages T3, T4 and phi X174 was studied in Escherichia coli cells frozen to -196 degrees C and cooled to 0 degree C at various intervals from the instant of phage infection. The processes of biosynthesis were delayed and the latent period was longer in the growth of cells frozen to -196 degrees C. The levels of RNA and protein biosynthesis as well as the yield of phages decreased when cells were frozen at a later stage of the phage growth. No changes were found in the intracellular growth processes of the phages during the subsequent cultivation of the bacterium when it was infected and then cooled to 0 degree C.