Adsorption of lambda phage DNA onto Escherichia coli cells treated with Ca2+ ions and onto frozen--thawed bacteria
Moiseeva, T.F.; Ditiatkin, S.I.; Kim, A.A.; Il'iashenko, B.N.
Biulleten' Eksperimental'noi Biologii i Meditsiny 89(3): 324-326
1980
ISSN/ISBN: 0365-9615 PMID: 6446330 Document Number: 162247
The study of the biologically active tritium-labeled phage lambda DNA adosrption on Ca2+-treated and frozen--thawed E. coli cells showed the absence of a correlation between the adsorption level and transfection efficiency. Thus the infectious phage lambda DNA adsorption level does not change, while frozing--thawing of E. coli cells but it increases ten-fold when treating the cells with Ca2+ in ice, the transfection efficiency level with this DNA being equal for both types of recipients.