Conditions of the synthesis of lysine decarboxylase by Escherichia coli MRE 600
Beretskene, S.Ia.; Bruzgulis, P.A.; Ragavichus, A.B.
Prikladnaia Biokhimiia i Mikrobiologiia 16(3): 351-355
1980
ISSN/ISBN: 0555-1099 PMID: 7001435 Document Number: 163341
The effect of aeration, pH of the cultivation medium, temperature and time of cultivation of Escherichia coli MRE600 on the synthesis of cellular lysine decarboxylase (LDC) was studied. It was demonstrated that LDC reached maximum activity in the 5-hour bacterial culture cultivated in the nutrient medium with pH 6.0 at 29--30 degrees C. Aeration increased the yield by about 3 times and decreased LDC activity by 5.5 times.