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Substrate specificity of uridine and purine nucleoside phosphorylases of the whole cells of Escherichia coli

Zintchenko, A.I.; Eroshevskaya, L.A.; Barai, V.N.; Mikhailopulo, I.A.

Nucleic Acids Symposium Series 18: 137-140

1987

ISSN/ISBN: 0261-3166
PMID: 3122186
Document Number: 297688
Substrate specificity of uridine and purine nucleoside phosphorylases of the whole cells of Escherichia coli BM-11 has been studied. Both enzymes reveal similar requirements to the structure and stereochemistry of uracil nucleosides and of the pentofuranose-1-phosphates, respectively, viz, a) modifications at C-3' decreased the substrate activity to a greater extent as compared with the same modifications at C-2'; b) substitution of a methyl group for one of the 5'-CH2 protons does not lead to essential alterations of the substrate activity of such analogs vs. the natural substrates - uridine and ribofuranose-1-phosphate, respectively. PNP exhibits a very broad specificity for the purine acceptor.

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Chebotarev, D.V.; Gul'ko, L.B.; Veĭko, V.P. 2001: Protein engineering of uridine phosphorylase from Escherichia coli K-12. II. Comparative study of hybrid and mutant forms of uridine phosphorylases Bioorganicheskaia Khimiia 27(3): 184-190
Cheng, N.; Payne, R.C.; Kemp, W.E.; Traut, T.W. 1986: Homogeneous uridine kinase from Ehrlich ascites tumor: substrate specificity and inhibition by bisubstrate analogs Molecular Pharmacology 30(2): 159-163
Strömberg, N.; Marklund, B.I.; Lund, B.; Ilver, D.; Hamers, A.; Gaastra, W.; Karlsson, K.A.; Normark, S. 1990: Host-specificity of uropathogenic Escherichia coli depends on differences in binding specificity to Gal alpha 1-4Gal-containing isoreceptors EMBO Journal 9(6): 2001-2010
Rosenkranz, H.S.; Carr, H.S. 1970: Hydroxyurea and Escherichia coli nucleoside diphosphate reductase Cancer Research 30(7): 1926-1927
Gol'tsmaĭer, T.A.; Mironov, A.S.; Sukhodolets, V.V. 1980: Effect of the ts 15 mutation of transcription termination factor rho on expression of the uridine phosphorylase gene of Escherichia coli Genetika 16(4): 597-601
Iuodka, B.A.; Labeĭkite, D.I.; Sasnauskene, S.I. 1993: Substrate specificity of T4 RNA-ligase: the role of a purine nucleotide base in forming a covalent AMP-RNA-ligase complex Biokhimiia 58(6): 857-865
Savva, D. 1980: Mutation in Escherichia coli during substrate-accelerated death Microbios 28(113-114)
Brikun, I.A.; Mironov, A.S.; Masiliunaĭte, R.V.; Sukhodolets, V.V. 1990: Cloning of Escherichia coli uridine phosphorylase gene: localization of structural and regulatory regions in the cloned fragment and identification of the protein product Molekuliarnaia Genetika Mikrobiologiia i Virusologiia 6: 7-11
Stevens, J.; Stevens, Y.W.; Hollander, V.P. 1974: Substrate requirements and kinetic analysis of the cortisol effects on uridine uptake and incorporation by mouse lymphoma P1798 cells in vitro Cancer Research 34(9): 2330-2337
Kanaya, S.; Kudo, Y. 1991: Specificity of promoter consensus sequences in Escherichia coli chromosome Nucleic Acids Symposium Series 1991(25): 41-42
Musier-Forsyth, K.; Stehlin, C.; Burke, B.; Liu, H. 1997: Understanding species-specific differences in substrate recognition by Escherichia coli and human prolyl-tRNA synthetases Nucleic Acids Symposium Series 1997(36): 5-7
Khristoforov, R.R.; Sukhareva, B.S.; Dixon, H.B.; Sparkes, M.J.; Krasnov, V.P.; Bukrina, I.M.; Grishakov, A.N. 1996: Reactions of decarboxylated and side transamination during interaction of glutamate decarboxylase from Escherichia coli with substrate analogs, modified through C3 and C4 atoms Biokhimiia 61(3): 464-471
Bruskov, V.I.; Kuklina, O.V. 1988: Display of 8-hydroxy-GTP substrate properties of UTP in the reaction of polynucleotide synthesis catalyzed by RNA-polymerase from Escherichia coli in the presence of poly[d(AT).d(AT)] template Molekuliarnaia Biologiia 22(3): 726-730
Timchenko, S.Kh.; Kubareva, E.A.; Volkov, E.M.; Voronina, O.L.; Lunin, V.G.; Gonchar, D.A.; Degtiarev, S.Kh.; Timchenko, M.A.; Kihara, H.; Kimura, K. 2006: Structure of Escherichia coli uracil DNA glycosylase and its complexes with nonhydrolyzable substrate analogues in solution observed by synchrotron small-angle X-ray scattering Biofizika 51(1): 5-12
Edberg, S.C.; Allen, M.J.; Smith, D.B. 1991: Defined substrate technology method for rapid and specific simultaneous enumeration of total coliforms and Escherichia coli from water: collaborative study Journal - Association of Official Analytical Chemists 74(3): 526-529
Schaaper, R.M. 1993: The mutational specificity of two Escherichia coli dnaE antimutator alleles as determined from lacI mutation spectra Genetics 134(4): 1031-1038
Tseng, C.C. 1981: Studies on pathogenic Escherichia coli. Part 8. Purification and properties of exotoxin from capsular polysaccharide-synthesizing Escherichia coli Journal of the Formosan Medical Association 80(9): 866-872
Moon, H.W.; Rogers, D.G.; Rose, R. 1988: Effects of an orally administered live Escherichia coli pilus vaccine on duration of lacteal immunity to enterotoxigenic Escherichia coli in swine American Journal of Veterinary Research 49(12): 2068-2071
Vernozy-Rozand, C. 1999: Verotoxin-producing Escherichia coli (VTEC) and Escherichia coli O157:H7 in medicine and food industry Annales de Biologie Clinique 57(5): 507-515
Maianskiĭ, A.N.; Molchanova, I.V.; Alatyrtseva, I.E. 1976: Allergenic fractions of bacteria belonging to the enteric family. VII. Characteristics of the intracellular proteins comprising the allergenic substrate of Escherichia coli using ion exchange chromatography Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 3: 28-32