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Guanylate cyclase in Escherichia coli. I. Purification of the enzyme and evidence for an inhibitor

Macchia, V.; Rocino, A.; Gulletta, E.; Consiglio, E.; Varrone, S.

Comptes Rendus des Seances de la Societe de Biologie et de ses Filiales 172(5): 860-867

1978

ISSN/ISBN: 0037-9026
PMID: 37997
Document Number: 138834
Guanylate cyclase activity is present in crude E. coli extract. Guanylate cyclase has been purified 3500 fold from this extract, through ammonium sulfate fractionation, DEAE-cellulose chromatography. Sephadex G-75 gel-filtration and polyacrylamide gel preparative microelectrophoresis. During the purification a guanylate cyclase inhibitor has been separated.

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